October 31, 2025 – We are pleased to announce the publication of our scientific article confirming the superior effectiveness of our patented high-pressure gas perfusion (HIPPER) technology in the preservation of donor hearts.
The research, titled "Improved Rat Heart Preservation Using High-Pressure Gaseous Perfusion with Oxygen-Xenon Mixture" has been published in the renowned, peer-reviewed journal, Pathophysiology. This publication marks a critical milestone, offering robust, independent validation of HIPPER as a revolutionary method poised to extend the viability of transplantable organs.
The Experiment: A Clear Superiority
The study utilized Wistar rat hearts to compare the conventional SCS method using HTK solution against the novel HIPPER technique. Hearts were preserved for a substantial period of six hours, followed by a one-hour Langendorff assessment to measure functional recovery.
The results unequivocally demonstrated the enhanced preservation capabilities of HIPPER, regardless of the gaseous mixture used:
Resuscitation Success: All 15 hearts preserved using the HIPPER method with oxygen-xenon mixtures were successfully resuscitated (15/15), compared to only 4 out of 10 in the Control (SCS) group.
Heart Rate: Experimental (Exp) hearts preserved with the oxygen-xenon mixture exhibited a significantly higher mean heart rate (mean 226±13 bpm) compared to both the Control (mean 131±10 bpm) and the Air (HIPPER using air) groups (mean 164±21 bpm).
Ventricular Function: Left ventricular developed pressure (LVDP) was also markedly higher in the Exp group (mean 73±7 mmHg) compared to the Control (mean 31±5 mmHg) and Air (mean 45±9 mmHg) groups.
Reduced Infarction: The HIPPER method significantly reduced ischemia-reperfusion injury, with the Exp group averaging only 6.3±0.7% infarct size of the total myocardium area, a drastic improvement over the Control group's 39.6±6.6%.
These quantitative and qualitative findings validate that HIPPER consistently outperformed SCS after six hours of storage, highlighting its potential as a more robust and effective preservation method.
HIPPER's Unique Position in Preservation Technology
While other preservation modalities exist—including static cold storage (SCS), hypothermic machine perfusion (HMP) and normothermic machine perfusion (NMP) — the HIPPER technology represents a distinct and powerful approach. The closest comparable technique, Persufflation (PSF), involves perfusion of organs with gaseous mixtures. However, HIPPER overcomes the key limitations of PSF with four critical innovations:
A hermetically sealed, pressurized chamber for organ placement.
A closed-loop gas perfusion system utilizing a pre-compressed gas mixture, which prevents open gas outflow.
The exclusion of fluidic preservation media, relying solely on elevated gaseous pressure.
A unique gaseous mixture containing Xenon (Xe) and Oxygen, leveraging Xe's protective properties.
This study emphatically demonstrates the efficacy of this uniquely designed system, validating its capacity to function as the sole driver of a closed gaseous perfusion circuit. This positions HIPPER as a robust, non-fluidic, closed-system alternative, highlighting its potential for broader translational adoption.
Acknowledging Scientific Excellence
"The publication in Pathophysiology is more than just a news item; it is an essential recognition by the global scientific community that our HIPPER technology represents a substantial and needed advancement in organ preservation science," said Arkadiy Ivanov, co-founder and Business Development Manager. () "The peer-review stamp of approval is the gold standard in biotechnology, lending immense credibility to the potential of HIPPER to fundamentally change transplant medicine, increase the donor organ pool, and save lives globally. We are committed to supporting further research and development to bring this solution to the clinical stage."
Limenbio Inc. is committed to supporting further research and development to bring the HIPPER technology to the clinical stage, aiming to increase the viability of donor organs and shorten transplant waiting lists.
The full article, "Improved Rat Heart Preservation Using High-Pressure Gaseous Perfusion with Oxygen-Xenon Mixture" by Alexandr Ponomarev et al., can be accessed here: